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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly is made up of the epitope identified from the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining from the blocked antibody versus the antibody by itself, you can see which staining is precise.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose customers check here are characterized by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which lead to your temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is degraded as cells progress through S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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